It's a Saturday night, and I'm in the lab.
I was feeling hopeful about today's attempt at isolating some plasmid DNA from bacteria, seeing that I was trying out a new protocol.
Well... my DNA yield on all my samples was pretty low. Nothing at this point seems to be working in my favor and I feel like my data isn't representative of the effort I have put into this project. To this date, I have three pieces of data.
Yep, that's it.
Not gonna lie, I feel like ditching this part of my thesis project but it is a crucial aspect to understanding the bigger picture. I feel like I'm taking all the necessary steps to make this project work, yet I have nothing to show for it. I'm trying to remain positive about all of this, because at the end of the day my failures are allowing me to refine my technique, and think more like a scientist.
Hopefully, next week will be better.
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